The effects of competition from non-pathogenic foodborne bacteria during the selective enrichment of Listeria monocytogenes using buffered Listeria enrichment broth
نویسندگان
چکیده
منابع مشابه
Rapid quantitative detection of Listeria monocytogenes in chicken using direct and combined enrichment/qPCR method
Listeria monocytogenes is a species of foodborne pathogen often related to foods, such as poultry, ready-to-eat products, fruits, and vegetables. The culture method is a standard procedure for the detection of bacteria in food products. The real-time quantitative PCR (qPCR) technique can be used for the quantification of foodborne pathogens. The current research was aimed to assess...
متن کاملSelective-enrichment procedure for isolation of Listeria monocytogenes from fecal and biologic specimens.
A selective-enrichment procedure (SEP) was developed to isolate Listeria monocytogenes from fecal and biologic specimens. This procedure was compared with direct plating with McBride listeria agar and 2-, 4-, and 8-week cold-enrichment procedures in recovering L. monocytogenes from mouse fecal, liver, and brain specimens. Although the SEP occasionally did not isolate the organism from specimens...
متن کاملListeria monocytogenes: a foodborne pathogen.
Listeriosis, caused by Listeria monocytogenes, appears to be increasing in incidence worldwide. The disease is of great concern to the food industry. A recent outbreak in California was linked to the consumption of Mexican-style soft cheese and involved more than 300 cases, 30% of which were fatal. L. monocytogenes can be found in a variety of dairy products, leafy vegetables, fish and meat pro...
متن کاملInhibitory effects of enrichment media on the Accuprobe test for Listeria monocytogenes.
During an evaluation of the Accuprobe kit for the detection of Listeria monocytogenes, some of the enrichment media used were found to interfere with the test. Microscopic examination during the lysis step of the test revealed that media containing high salt greatly reduced or prevented cell lysis. This prevented the probe from binding to the cellular RNA, resulting in false-negative results.
متن کاملEvaluation of a novel Listeria enrichment broth combined with a realtime PCR diagnostics assay for the specific detection of Listeria monocytogenes in RTE pork products
Foodborne outbreaks of listeriosis, human infection with Listeria monocytogenes, are relatively uncommon with approximately 0.1–1.1 cases /100 000 population globally, but high mortality rates of 20–30% are associated with infection (Siegman-Igra et al., 2002; Anonymous, 2012). Those at particular risk of infection include the immunocomprised, the elderly, neonates and pregnant women (Anonymous...
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ژورنال
عنوان ژورنال: Food Microbiology
سال: 2014
ISSN: 0740-0020
DOI: 10.1016/j.fm.2014.05.004